| ORIGINAL ARTICLE |
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| Year : 2016 | Volume
: 2
| Issue : 4 | Page : 195-202 |
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A Gold Nanoparticle-enhanced Surface Plasmon Resonance Aptasensor for the Detection of 2,4,6-trinitrotoluene
Junpeng Tan1, Bin Hao2, Chuo Wang2, Yifei Ren1, Hongxia Hao2, Ruiqin Yang3
1 Collaborative Innovation Center of Judicial Civilization; People's Public Security University of China, Beijing 100038, China
2 Collaborative Innovation Center of Judicial Civilization; Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing 100088, China
3 People's Public Security University of China, Beijing 100038, China
Correspondence Address:
Hongxia Hao
Collaborative Innovation Center of Judicial Civilization, Beijing 100088; Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing 100088
China
Ruiqin Yang
People's Public Security University of China, Beijing 100038
China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/2349-5014.197934
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A gold nanoparticle-enhanced surface plasmon resonance (SPR) aptasensor was developed for high-specificity and high-sensitivity detection of 2,4,6-trinitrotoluene (TNT). Self-assembly film-forming technology was used to modify the gold surface of the sensor chip with 2,4,6-trinitrophenyl glycine, a TNT analogue, using polyethylene glycol to which the thiol group and carboxyl group are attached. Aptamer-gold nanoparticle complexes were formed through Au-S bonding. To detect TNT, the samples were incubated with the aptamer-gold nanoparticle complexes, and the solution competition method was applied through the SPR aptasensor. The results showed that the SPR aptasensor achieved fast, real-time detection of TNT. This gold nanoparticle-enhanced SPR aptasensor is suitable for TNT detection in the field of public safety and environmental monitoring. |
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