| BRIEF COMMUNICATION |
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| Year : 2022 | Volume
: 8
| Issue : 2 | Page : 76-79 |
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The impact of different stain carriers on the mrna profiling from bloodstains
Hemiao Zhao1, Qingluan Lin2, Qi Zhang3, Jing Chen1, Zheng Tu1, Ruiqin Yang3, Lan Hu1, Chong Wang1
1 Division of Forensic Genetics, Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, China
2 Qionghai Police Bureau, Hainan Province, China
3 School of Crime Investigation, Chinese People's Public Security University, Beijing, China
Correspondence Address:
Chong Wang
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100 038
China
Lan Hu
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100 038
China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/jfsm.jfsm_36_21
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Unlike DNA profiling, mRNA profiling is greatly affected by external factors. To analyze the influence of different stain carriers on the detectability of mRNA markers from bloodstains, this study examined 10 carriers, including a knife, cotton swab, paper, plastic, leather, cement, chopsticks, clothes, ceramic block, and wall. After detecting five specific mRNA markers (HBA, HBB, ALAS2, GYPA, and SPTB) and the housekeeping gene B2M in peripheral blood samples, no statistically significant differences in the effects of the carriers were found. The results suggest that when performing mRNA testing on bloodstains, the effect of the stain carrier has little influence.
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